Thrombospondin-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42522

Novus Biologicals, part of Bio-Techne
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NBP2-42522
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Thrombospondin-1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence NRIPESGGDNSVFD-C corresponding to N-Terminus according to NP_003237.2.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Thrombospondin-1 Antibody

Western Blot: Thrombospondin-1 Antibody [NBP2-42522]

Western Blot: Thrombospondin-1 Antibody [NBP2-42522]

Western Blot: Thrombospondin-1 Antibody [NBP2-42522] - (1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence
Immunocytochemistry/ Immunofluorescence: Thrombospondin-1 Antibody [NBP2-42522]

Immunocytochemistry/ Immunofluorescence: Thrombospondin-1 Antibody [NBP2-42522]

Immunocytochemistry/Immunofluorescence: Thrombospondin-1 Antibody [NBP2-42522] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody [NBP2-42522]

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody [NBP2-42522]

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody [NBP2-42522] - (5ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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Applications for Thrombospondin-1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3-1 ug/ml
Application Notes
Western Blot: Approx 150kDa band observed in Mouse Kidney lysates (calculated MW of 129kDa according to NP_035710.2). Primary incubation 1 hour at room temperature.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and HepG2 cells.
Flow Cytometry: Flow cytometric analysis of A431 cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Thrombospondin-1

Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Alternate Names

THBS1, Thrombospondin1, TSP-1

Entrez Gene IDs

7057 (Human)

Gene Symbol

THBS1

Additional Thrombospondin-1 Products

Product Documents for Thrombospondin-1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Thrombospondin-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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