Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25719

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25719
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1140

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Thrombospondin-2 Antibodies »

Product Summary for Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free

Immunogen

Recombinant protein encompassing a sequence within the center region of human Thrombospondin-2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free

Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free

Western Blot: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] -

Western Blot: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - AsPC-1 whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] -

Immunocytochemistry/Immunofluorescence: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Thrombospondin 2 antibody [HL1140] detects Thrombospondin 2 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:500. Blue: Fluoroshield with DAPI .
Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free

Immunohistochemistry-Paraffin: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] -

Immunohistochemistry-Paraffin: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Thrombospondin 2 antibody [HL1140] detects Thrombospondin 2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human pancreatic cancer. Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free

Application
Recommended Usage

ELISA

Assay dependent

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Sandwich ELISA

Assay dependent

Western Blot

Assay dependent
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thrombospondin-2

Thrombospondin 2 is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. It can bind to fibrinogen, fibronectin, laminin and type V collagen. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration.

Alternate Names

THBS2, Thrombospondin2, TSP-2

Gene Symbol

THBS2

Additional Thrombospondin-2 Products

Product Documents for Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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