Thrombospondin-3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36996

Novus Biologicals, part of Bio-Techne
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NBP1-36996
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Thrombospondin-3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CNDTVPEDFEPFRRQ corresponding to C-Terminus according to NP_009043.1.

Epitope

C Terminus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Thrombospondin-3 Antibody

Immunohistochemistry-Paraffin: Thrombospondin-3 Antibody [NBP1-36996]

Immunohistochemistry-Paraffin: Thrombospondin-3 Antibody [NBP1-36996]

Immunohistochemistry-Paraffin: Thrombospondin-3 Antibody [NBP1-36996] - Staining of Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Thrombospondin-3 Antibody

Application
Recommended Usage

Immunohistochemistry

1 - 2 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 50 kDa band in human lung and in lysates of A549 after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104 kDa band according to NP_009043.1. The 50 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human colon shows endothelial cell staining of a blood vessel.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Thrombospondin-3

THBS3 is a gene that codes for an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and has two isoforms, with lengths of 956 and 836 amino acids and weights of approximately 104 and 91 kDa respectively. Current research is being done on diseases and disorders related to this gene including osteosarcoma, lung cancer, breast cancer, retinitis, malaria, neuronitis, and echinococcosis. THBS3 has also been shown to have interactions with THBS2, THBS1, THBS4, FURIN, and PDGFB in pathways such as the focal adhesion, inflammatory response, signal transduction, signaling by PDGF, phagosome, ECM-receptor interaction, and malaria pathways.

Alternate Names

THBS3, Thrombospondin3, TSP3

Entrez Gene IDs

7059 (Human); 21827 (Mouse)

Gene Symbol

THBS3

UniProt

NP_009043.1 (Human)

Additional Thrombospondin-3 Products

Product Documents for Thrombospondin-3 Antibody

Product Datasheets

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Product Specific Notices for Thrombospondin-3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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