Thymidine Kinase 1 Antibody (3F9)

Novus Biologicals, part of Bio-Techne | Catalog # H00007083-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3F9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Thymidine Kinase 1 Antibodies »

Product Specifications

Immunogen

TK1 (AAH07986.1, 1 a.a. ~ 234 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFFPDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAARKLFAPQQILQCSPAN

Specificity

TK1 (3F9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Thymidine Kinase 1 Antibody (3F9)

Western Blot: Thymidine Kinase 1 Antibody (3F9) [H00007083-M05]

Western Blot: Thymidine Kinase 1 Antibody (3F9) [H00007083-M05]

Western Blot: Thymidine Kinase 1 Antibody (3F9) [H00007083-M05] - Analysis of TK1 expression in transfected 293T cell line by TK1 monoclonal antibody (M05), clone 3F9. Lane 1: TK1 transfected lysatE (25.469 KDa). Lane 2: Non-transfected lysate.
ELISA: Thymidine Kinase 1 Antibody (3F9) [H00007083-M05]

ELISA: Thymidine Kinase 1 Antibody (3F9) [H00007083-M05]

ELISA: Thymidine Kinase 1 Antibody (3F9) [H00007083-M05] - Detection limit for recombinant GST tagged TK1 is approximately 1ng/ml as a capture antibody.

Applications for Thymidine Kinase 1 Antibody (3F9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Thymidine Kinase 1

Thymidine kinase (TK) belongs to a group of enzymes such as dihydrofolate reductase, thymidylate synthase, and DNA polymerase that are involved in DNA synthesis and precursor production. Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells due to multiple regulatory mechanisms that ensure expression of these enzymes exclusively in replicating cells. TK is responsible for catalyzing the phosphorylation of thymidine, which functions as a part of the pyrimidine salvage pathway involved in DNA synthesis. The activities of enzymes such as TK are essential for the activation of several chemotherapeutically important nucleoside analogues that are administered as prodrugs.

Alternate Names

TK1

Entrez Gene IDs

7083 (Human)

Gene Symbol

TK1

OMIM

188300 (Human)

UniProt

AAH07986 (Human)

Additional Thymidine Kinase 1 Products

Product Documents for Thymidine Kinase 1 Antibody (3F9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Thymidine Kinase 1 Antibody (3F9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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