Thymidine Kinase 1 Antibody (6F6)

Western Blot: Thymidine Kinase 1 Antibody (6F6) [NBP3-26286] -

Western Blot: Thymidine Kinase 1 Antibody (6F6) [NBP3-26286] - Positive Western Blot detected in: 293T whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate.
All lanes: Thymidine Kinase 1 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 26 kDa
Observed band size: 26 kDa

Immunocytochemistry/Immunofluorescence: Thymidine Kinase 1 Antibody (6F6) [NBP3-26286] -

Immunocytochemistry/Immunofluorescence: Thymidine Kinase 1 Antibody (6F6) [NBP3-26286] - Staining of Hela with Thymidine Kinase 1 Antibody (6F6) at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 491-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: Thymidine Kinase 1 Antibody (6F6) [NBP3-26286] -

Flow Cytometry: Thymidine Kinase 1 Antibody (6F6) [NBP3-26286] - Overlay Peak curve showing Hela cells stained with Thymidine Kinase 1 Antibody (6F6) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.