Thymidine Kinase 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-60644

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Thymidine Kinase 1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 205 and 234 of human thymidine kinase 1 using the numbering given in entry NP_003249.1 (GeneID 7083).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Thymidine Kinase 1 Antibody

Western Blot: Thymidine Kinase 1 Antibody [NB100-60644]

Western Blot: Thymidine Kinase 1 Antibody [NB100-60644]

Western Blot: Thymidine Kinase 1 Antibody [NB100-60644] - Detection of Human TK1 on HeLa whole cell lysate using NB100-60644. TK1 was also immunoprecipitated by rabbit anti-TK1 antibody NB100-60643.

Applications for Thymidine Kinase 1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Thymidine Kinase 1

Thymidine kinase (TK) belongs to a group of enzymes such as dihydrofolate reductase, thymidylate synthase, and DNA polymerase that are involved in DNA synthesis and precursor production. Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells due to multiple regulatory mechanisms that ensure expression of these enzymes exclusively in replicating cells. TK is responsible for catalyzing the phosphorylation of thymidine, which functions as a part of the pyrimidine salvage pathway involved in DNA synthesis. The activities of enzymes such as TK are essential for the activation of several chemotherapeutically important nucleoside analogues that are administered as prodrugs.

Alternate Names

TK1

Entrez Gene IDs

7083 (Human)

Gene Symbol

TK1

UniProt

P04183

Additional Thymidine Kinase 1 Products

Product Documents for Thymidine Kinase 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Thymidine Kinase 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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