Thymidine Kinase 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20626

Novus Biologicals, part of Bio-Techne
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NBP2-20626
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Predicted:

Bovine (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Thymidine Kinase 1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Thymidine Kinase 1. The exact sequence is proprietary.

Reactivity Notes

Chicken (83%), Zebrafish (81%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Thymidine Kinase 1 Antibody

Western Blot: Thymidine Kinase 1 Antibody [NBP2-20626]

Western Blot: Thymidine Kinase 1 Antibody [NBP2-20626]

Western Blot: Thymidine Kinase 1 Antibody [NBP2-20626] - Wild-type (WT) and Thymidine Kinase 1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase 1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody
Immunohistochemistry-Paraffin: Thymidine Kinase 1 Antibody [NBP2-20626]

Immunohistochemistry-Paraffin: Thymidine Kinase 1 Antibody [NBP2-20626]

Immunohistochemistry-Paraffin: Thymidine Kinase 1 Antibody [NBP2-20626] - Thymidine Kinase 1 antibody detects Thymidine Kinase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat ovary. Thymidine Kinase 1 stained by Thymidine Kinase 1 antibody diluted at 1:500.
Western Blot: Thymidine Kinase 1 Antibody [NBP2-20626]

Western Blot: Thymidine Kinase 1 Antibody [NBP2-20626]

Western Blot: Thymidine Kinase 1 Antibody [NBP2-20626] - A431 whole cell and cytoplasm extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase 1 antibody diluted at 1:1000.
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Applications for Thymidine Kinase 1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Thymidine Kinase 1

Thymidine kinase (TK) belongs to a group of enzymes such as dihydrofolate reductase, thymidylate synthase, and DNA polymerase that are involved in DNA synthesis and precursor production. Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells due to multiple regulatory mechanisms that ensure expression of these enzymes exclusively in replicating cells. TK is responsible for catalyzing the phosphorylation of thymidine, which functions as a part of the pyrimidine salvage pathway involved in DNA synthesis. The activities of enzymes such as TK are essential for the activation of several chemotherapeutically important nucleoside analogues that are administered as prodrugs.

Alternate Names

TK1

Gene Symbol

TK1

UniProt

P04183

Additional Thymidine Kinase 1 Products

Product Documents for Thymidine Kinase 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Thymidine Kinase 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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