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Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25722

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25722

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine, Drosophila, Feline

Predicted:

Chimpanzee (99%), Porcine (95%), Rat (94%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1237

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Thymidylate Synthase. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (86%), Xenopus (84%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free

Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free

Western Blot: Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free [NBP3-25722] -

Western Blot: Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free [NBP3-25722] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1237] (NBP3-25722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free

Western Blot: Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free [NBP3-25722] -

Western Blot: Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free [NBP3-25722] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1237] (NBP3-25722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free

Western Blot: Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free [NBP3-25722] -

Western Blot: Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free [NBP3-25722] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1237] (NBP3-25722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thymidylate Synthase

Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.

Long Name

Thymidylate synthase

Alternate Names

EC 2.1.1.45, OK/SW-cl.29, TS, TSase, TYMS

Gene Symbol

TYMS

Additional Thymidylate Synthase Products

Product Documents for Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Thymidylate Synthase Antibody (HL1237) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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