Thymidylate Synthase Antibody (TYMS/1884) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08882

Novus Biologicals, part of Bio-Techne
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NBP3-08882
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # TYMS/1884

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Thymidylate Synthase Antibodies »

Product Specifications

Immunogen

Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary) (Uniprot: P04818)

Localization

Nuclear & Cytoplasmic

Marker

5-FU Resistance Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP3-07144).

Antibody with azide - store at 2 to 8C.

Scientific Data Images for Thymidylate Synthase Antibody (TYMS/1884) - Azide and BSA Free

Western Blot: Thymidylate Synthase Antibody (TYMS/1884)Azide and BSA Free [NBP3-08882]

Western Blot: Thymidylate Synthase Antibody (TYMS/1884)Azide and BSA Free [NBP3-08882]

Western Blot: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-08882] - Western Blot Analysis of human HeLa cell lysate using Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884).
Immunocytochemistry/ Immunofluorescence: Thymidylate Synthase Antibody (TYMS/1884) - Azide and BSA Free [NBP3-08882]

Immunocytochemistry/ Immunofluorescence: Thymidylate Synthase Antibody (TYMS/1884) - Azide and BSA Free [NBP3-08882]

Immunocytochemistry/Immunofluorescence: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-08882] - Immunofluorescence Analysis of PFA-fixed Ramos cells labeling PU.1 with Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884) followed by goat anti- Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin (Red).
Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TYMS/1884) - Azide and BSA Free [NBP3-08882]

Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TYMS/1884) - Azide and BSA Free [NBP3-08882]

Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-08882] - Formalin-fixed, paraffin-embedded human Tonsil stained with Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884).
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Applications for Thymidylate Synthase Antibody (TYMS/1884) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Thymidylate Synthase

Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.

Long Name

Thymidylate synthase

Alternate Names

EC 2.1.1.45, OK/SW-cl.29, TS, TSase, TYMS

Gene Symbol

TYMS

Additional Thymidylate Synthase Products

Product Documents for Thymidylate Synthase Antibody (TYMS/1884) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Thymidylate Synthase Antibody (TYMS/1884) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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