Thymine Dimer Antibody (H3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1141

Novus Biologicals, part of Bio-Techne
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NB600-1141
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Key Product Details

Species Reactivity

Validated:

Human, Chicken, Non-species specific

Cited:

Human, Mouse

Applications

Validated:

Dot Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # H3

Format

BSA Free

Concentration

2.0 mg/ml

View all Thymine Dimer Antibodies »

Product Specifications

Immunogen

tetra nucleotide containing cyclobutane thymine dimer (GTTG) conjugated to chicken gamma globulin.

Specificity

Monoclonal Anti-Thymine Dimer reacts specifically with the (5'-6') cyclobutane type of homothymine or thyminecytosine heterodimers. The antibody reacts with thymine dimers in single-stranded DNA, and has a lower affinity for the dimer in short oligonucleotides (a tail of minimum 10-20 thymine residues is required for efficient labeling of oligonucleotide probes). The product enables a sensitive and non-radioactive method for labeling, detection, and quantification of DNA fragments using ELISA, competitive ELISA, immunocytochemistry (laser-scan microscopy) and Southern immunoblotting.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Thymine Dimer Antibody (H3) - BSA Free

Immunocytochemistry/ Immunofluorescence: Thymine Dimer Antibody (H3) - BSA Free [NB600-1141]

Immunocytochemistry/ Immunofluorescence: Thymine Dimer Antibody (H3) - BSA Free [NB600-1141]

Immunocytochemistry/Immunofluorescence: Thymine Dimer Antibody (H3) [NB600-1141] - analysis of Thymine Dimer in human keratinocytes using anti-Thymine Dimer antibody. Image from verified customer review.

Applications for Thymine Dimer Antibody (H3) - BSA Free

Application
Recommended Usage

Dot Blot

0.5-1 ug/ml

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000
Application Notes
Southern Blotting, use UV induced thymine dimers of lamda phage DNA fragments
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB600-1141 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

2.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Thymine Dimer

Non-radioactive labeling of DNA is typically based on the enzymatic incorporation of modified nucleotides, carrying a small chemical moiety such as biotin, digoxigenin or fluorescein. These tags are subsequently detected by specific reagents such as streptavidin or a specific antibody coupled to a signal-producing enzyme. Although very efficient and reliable, labeling by in vitro polymerization is time-consuming, expensive, and may require various post-label purification steps to remove an excess of unincorporated precursors. An alternative strategy for DNA labeling, is based on the UV-induced formation of cyclobutane thymine dimers. Several methods have been described for the detection of thymine dimers, which are based on chromatographic analysis, and on biochemical analysis with endonucleases specific for UV-irradiated DNA. In addition, methods utilizing antibodies specific for pyrimidine dimers and other UV-induced DNA lesions have evolved, which permit the study of the induction and repair of these lesions without the requirement of in vivo radiolabeling of DNA. Photoimmunodetection is a rapid, reliable and low-cost supplement to existing methods for nonradioactive DNA labeling. It enables a sensitive and non-radioactive method for labeling, detection, and quantification of high molecular weight (HMW) DNA fragments. The method is based on the introduction of thymine dimers into DNA after separation by pulse field gel electrophoresis (PFGE), followed by detection with thymine dimer specific antibodies. This method does not require any enzymatic or chemical manipulation of the DNA sample. Monoclonal antibodies reacting specifically with thymine dimer facilitate investigations on the apoptotic process and the role of UV-induced pyrimidine dimers in the process of photocarcinogenesis.

Additional Thymine Dimer Products

Product Documents for Thymine Dimer Antibody (H3) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Thymine Dimer Antibody (H3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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