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Thyroid Peroxidase Antibody (JE60-40)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33016

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-33016

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE60-40

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant protein within Human Thyroid Peroxidase aa 834-933 / 933. (Uniprot: P07202)

Localization

Membrane

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Thyroid Peroxidase Antibody (JE60-40)

Thyroid Peroxidase Antibody (JE60-40)

Immunohistochemistry: Thyroid Peroxidase Antibody (JE60-40) [NBP3-33016]

Immunohistochemistry: Thyroid Peroxidase Antibody (JE60-40) [NBP3-33016] - Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-Thyroid Peroxidase antibody (NBP3-33016) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33016) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for Thyroid Peroxidase Antibody (JE60-40)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thyroid Peroxidase

Thyroid Peroxidase encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Additional splice variants have been described but their biological natures have not been determined. [provided by RefSeq]

Alternate Names

EC 1.11.1, MSA, TDH2A, thyroid microsomal antigen, thyroid peroxidase, thyroperoxidase, TPXEC 1.11.1.8

Gene Symbol

TPO

Additional Thyroid Peroxidase Products

Product Documents for Thyroid Peroxidase Antibody (JE60-40)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Thyroid Peroxidase Antibody (JE60-40)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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