TIAL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49832

Novus Biologicals, part of Bio-Techne
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NBP1-49832
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all TIAL1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SPEITTEDIKSAFAP corresponding to internal region according to NP_003243.1, NP_001029097.1.

Epitope

SPEITTEDIKSAFAP - Internal region

Specificity

This antibody is expected to recognize both isoforms (NP_003243.1; NP_001029097.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TIAL1 Antibody

Western Blot: TIAL1 Antibody [NBP1-49832]

Western Blot: TIAL1 Antibody [NBP1-49832]

Western Blot: TIAL1 Antibody [NBP1-49832] - (2ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TIAL1 Antibody [NBP1-49832]

Immunohistochemistry-Paraffin: TIAL1 Antibody [NBP1-49832]

Immunohistochemistry-Paraffin: TIAL1 Antibody [NBP1-49832] - Staining of Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for TIAL1 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/ml

Immunohistochemistry-Paraffin

3-5 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 45 kDa band observed in K562 lysates (calculated MW of 43.4 kDa band according to NP_001029097.1). IHC-P: Human skin shows staining of select keratinocytes.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TIAL1

The protein encoded by this gene is a member of a family of RNA-binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing.

Alternate Names

aging-associated gene 7 protein, MGC33401, TCBP, T-cluster binding protein, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TIA1 cytotoxic granule-associated RNA-binding protein-like 1, TIA-1 related protein, TIA-1-related nucleolysin, TIA-1-related protein, TIARnucleolysin TIAR

Entrez Gene IDs

7073 (Human); 21843 (Mouse)

Gene Symbol

TIAL1

UniProt

NP_003243.1 (Human)

Additional TIAL1 Products

Product Documents for TIAL1 Antibody

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Certificate of Analysis

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Product Specific Notices for TIAL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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