Tie-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20635
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Tie-1 Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Tie-1. The exact sequence is proprietary.
Localization
Membrane; Single-pass type I membrane protein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Tie-1 Antibody
![Western Blot: Tie-1 Antibody [NBP2-20635]](https://resources.bio-techne.com/images/products/Tie-1-Antibody-Western-Blot-NBP2-20635-img0008.jpg "Western Blot: Tie-1 Antibody [NBP2-20635]")
Western Blot: Tie-1 Antibody [NBP2-20635]
Western Blot: Tie-1 Antibody [NBP2-20635] - Analysis. A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug K562 whole cell lysate/extract 5 % SDS-PAGE dilution: 1:1000![Immunohistochemistry-Paraffin: Tie-1 Antibody [NBP2-20635]](https://resources.bio-techne.com/images/products/Tie-1-Antibody-Immunohistochemistry-Paraffin-NBP2-20635-img0004.jpg "Immunohistochemistry-Paraffin: Tie-1 Antibody [NBP2-20635]")
Immunohistochemistry-Paraffin: Tie-1 Antibody [NBP2-20635]
Immunohistochemistry-Paraffin: Tie-1 Antibody [NBP2-20635] - Human colon carcinoma, using TIE1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.![Western Blot: Tie-1 Antibody [NBP2-20635]](https://resources.bio-techne.com/images/products/Tie-1-Antibody-Western-Blot-NBP2-20635-img0007.jpg "Western Blot: Tie-1 Antibody [NBP2-20635]")
Western Blot: Tie-1 Antibody [NBP2-20635]
Western Blot: Tie-1 Antibody [NBP2-20635] - A. 30 ug 293T whole cell lysate/extractB. 30 ug whole cell lysate/extract of human Tie-1-transfected 293T cells5 % SDS-PAGE Tie-1 antibody dilution: 1:5000.Applications for Tie-1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Tie-1
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 1
Alternate Names
Tie1
Gene Symbol
TIE1
UniProt
Additional Tie-1 Products
Product Documents for Tie-1 Antibody
Product Specific Notices for Tie-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.