Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all TIF1 alpha Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 706-1016 of human TRIM24 (NP_003843) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TIF1 alpha Antibody (OTI2D9) - Azide and BSA Free

Western Blot: TIF1 alpha Antibody (OTI2D9)Azide and BSA Free [NBP2-74514]

Western Blot: TIF1 alpha Antibody (OTI2D9)Azide and BSA Free [NBP2-74514]

Western Blot: TIF1 alpha Antibody (OTI2D9) - Azide and BSA Free [NBP2-74514] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIF1.
Immunohistochemistry: TIF1 alpha Antibody (OTI2D9) - Azide and BSA Free [NBP2-74514]

Immunohistochemistry: TIF1 alpha Antibody (OTI2D9) - Azide and BSA Free [NBP2-74514]

Immunohistochemistry: TIF1 alpha Antibody (OTI2D9) - Azide and BSA Free [NBP2-74514] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: TIF1 alpha Antibody (OTI2D9) - Azide and BSA Free [NBP2-74514]

Immunohistochemistry: TIF1 alpha Antibody (OTI2D9) - Azide and BSA Free [NBP2-74514]

Immunohistochemistry: TIF1 alpha Antibody (OTI2D9) - Azide and BSA Free [NBP2-74514] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for TIF1 alpha Antibody (OTI2D9) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TIF1 alpha

TIF1 alpha is encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Long Name

Transcription intermediary factor 1-alpha

Alternate Names

RNF82, TIF1, TIF1-alpha, TIF1A, TRIM24

Gene Symbol

TRIM24

Additional TIF1 alpha Products

Product Documents for TIF1 alpha Antibody (OTI2D9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TIF1 alpha Antibody (OTI2D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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