TIF1 gamma Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83747

Novus Biologicals, part of Bio-Techne
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NBP1-83747
NBP1-83747-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TIF1 gamma Antibodies »

Product Summary for TIF1 gamma Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MQPHLQRQHSNPGHAGPFPVVSVHNTTINPTSPTTATMANANRGPTSPSVTAIELIPSVTNPENLPSLPDIPPIQLEDAGSSSLDNLLSRYISGSHLPPQPTSTMNPSPGPSALSPGSSGLSNSHTPVRPPSTSSTGSR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TIF1 gamma Antibody

Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP1-83747]

Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP1-83747]

Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP1-83747] - Staining in human placenta and skeletal muscle tissues using NBP1-83747 antibody. Corresponding TRIM33 RNA-seq data are presented for the same tissues.
Western Blot: TIF1 gamma Antibody [NBP1-83747]

Western Blot: TIF1 gamma Antibody [NBP1-83747]

Western Blot: TIF1 gamma Antibody [NBP1-83747] - Analysis in human cell lines MCF-7 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: TIF1 gamma Antibody [NBP1-83747]

Immunocytochemistry/ Immunofluorescence: TIF1 gamma Antibody [NBP1-83747]

Immunocytochemistry/Immunofluorescence: TIF1 gamma Antibody [NBP1-83747] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
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Applications for TIF1 gamma Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TIF1 gamma

TIF1 gamma is encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined.

Alternate Names

E3 ubiquitin-protein ligase TRIM33, EC 6.3.2, EC 6.3.2.-, Ectodermin homolog, KIAA1113, Protein Rfg7, PTC7FLJ32925, RET-fused gene 7 protein, RFG7FLJ11429, TF1G, TIF1GAMMA, TIF1-gamma, TIF1GECTO, TIFGAMMA, Transcription intermediary factor 1-gamma, transcriptional intermediary factor 1 gamma, tripartite motif containing 33, tripartite motif-containing 33, Tripartite motif-containing protein 33

Gene Symbol

TRIM33

Additional TIF1 gamma Products

Product Documents for TIF1 gamma Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TIF1 gamma Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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