Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.5 mg/ml

View all TIGAR/C12orf5 Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an internal region of the human TIGAR protein (within residues 50-200). [Swiss-Prot Q9NQ88]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TIGAR/C12orf5 Antibody - BSA Free

Western Blot: TIGAR/C12orf5 AntibodyBSA Free [NBP1-49534]

Western Blot: TIGAR/C12orf5 AntibodyBSA Free [NBP1-49534]

Western Blot: TIGAR/C12orf5 Antibody [NBP1-49534] - Analysis of TIGAR in HeLa whole cell extracts.
Immunohistochemistry: TIGAR/C12orf5 Antibody - BSA Free [NBP1-49534]

Immunohistochemistry: TIGAR/C12orf5 Antibody - BSA Free [NBP1-49534]

Immunohistochemistry: TIGAR/C12orf5 Antibody [NBP1-49534] - Analysis of TIGAR in mouse muscle.

Applications for TIGAR/C12orf5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:5000
Application Notes
This TIGAR antibody is useful for Immunohistochemistry-paraffin sections and Western blot. In Western blot bands are seen ~30 kDa, representing TIGAR, and ~47 kDa. At this time we cannot explain the band at 47 kDa. This antibody does not appear to work in ICC.

Reviewed Applications

Read 1 review rated 5 using NBP1-49534 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TIGAR/C12orf5

TIGAR (TP53-induced glycolysis and apoptosis regulator), a member of phosphoglycerate mutase family, is a target gene of p53 and it regulates glucose metabolism in cancer cells by acting as a fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate (Fru-2,6-P2 ) and Fru-1,6-P2 leading to decreased Fru-2,6-P2 levels with subsequent reduction in PFK1 (phosphofructosekinase-1) activity as well as negative regulation of glycolysis. TIGAR also reduces cellular ROS through its translocation to the mitochondria where it interacts with HK2 leading to enhanced HK2 activity, regulation of mitochondrial membrane potential, and decreased mitochondrial ROS which ultimately protects the cells from DNA damage-induced apoptosis. Moreover, TIGAR mediated negative regulation of glycolysis leads to redirection of glycolytic metabolic intermediates towards pentose phosphate pathway's oxidative branch that follows increased cellular NADPH production, ROS scavenging by GSH and thus a lower sensitivity of cells to oxidative stress-associated apoptosis, including that induced by p53. Because of TIGAR's anti-apoptotic/protective role, it has been proposed to implicate in cell-cycle arrest and DNA repair effector response in cells that suffer from repairable dose of genotoxic/cellular insult.

Long Name

TP53-induced Glycolysis and Apoptosis Regulator

Alternate Names

C12orf5

Entrez Gene IDs

57103 (Human); 319801 (Mouse)

Gene Symbol

TIGAR

UniProt

Q9NQ88 (Human), Q8BZA9 (Mouse)

Additional TIGAR/C12orf5 Products

Product Documents for TIGAR/C12orf5 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TIGAR/C12orf5 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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