Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25773

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25773
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Canine (95%), Mouse (95%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1133

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Tight Junction Protein 1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Tight Junction Protein 1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

195 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free

Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free

Western Blot: Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free [NBP3-25773] -

Western Blot: Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free [NBP3-25773] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1133] (NBP3-25773) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free [NBP3-25773] -

Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free [NBP3-25773] - ZO-1 antibody [HL1133] detects ZO-1 protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZO-1 stained by ZO-1 antibody [HL1133] (NBP3-25773) diluted at 1:500. Blue: Fluoroshield with DAPI .
Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free

Knockout Validated: Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free [NBP3-25773] -

Knockout Validated: Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free [NBP3-25773] - Wild-type (WT) and TJP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1133] (NBP3-25773) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tight Junction Protein 1

Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.

Alternate Names

DKFZp686M05161, MGC133289, Tight junction protein 1, tight junction protein 1 (zona occludens 1), tight junction protein ZO-1, TJP1, ZO1, ZO-1, zona occludens 1, Zona occludens protein 1, zonula occludens 1 protein, Zonula occludens protein 1

Gene Symbol

TJP1

Additional Tight Junction Protein 1 Products

Product Documents for Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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