Tight Junction Protein 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46111
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Peptide ELISA
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-PKPTSQNQFSEHDKT corresponding to internal region according to NP_003248.3, NP_783297.2.
Epitope
internal region
Specificity
This antibody is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2).
Marker
Intercellular Junctions/Tight Junction Marker
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Tight Junction Protein 1 Antibody
Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] -
Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cellular junction staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] -
Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).Applications for Tight Junction Protein 1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:132000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Tight Junction Protein 1
Alternate Names
DKFZp686M05161, MGC133289, Tight junction protein 1, tight junction protein 1 (zona occludens 1), tight junction protein ZO-1, TJP1, ZO1, ZO-1, zona occludens 1, Zona occludens protein 1, zonula occludens 1 protein, Zonula occludens protein 1
Entrez Gene IDs
7082 (Human)
Gene Symbol
TJP1
UniProt
Additional Tight Junction Protein 1 Products
Product Documents for Tight Junction Protein 1 Antibody
Product Specific Notices for Tight Junction Protein 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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