Tight Junction Protein 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49669

Novus Biologicals, part of Bio-Techne
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NBP1-49669-0.025mg
NBP1-49669-0.05mg
NBP1-49669-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Guinea Pig IgG

Concentration

0.1 mg/ml

View all Tight Junction Protein 1 Antibodies »

Product Specifications

Immunogen

The polyclonal antibody is raised against a human peptide antigen corresponding to ten residues at the splice junction and as such recognizes specifically ZO-1 alpha-minus.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23871805).

Specificity

The polyclonal antibody is raised against a human peptide antigen corresponding to ten residues at the splice junction and as such recognizes specifically ZO-1 alpha-minus.

Marker

Intercellular Junctions/Tight Junction Marker

Clonality

Polyclonal

Host

Guinea Pig

Isotype

IgG

Applications for Tight Junction Protein 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For immunohistology, immunofluorescence and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, containing 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Tight Junction Protein 1

The polyclonal antibody recognizes human zona occludens 1 (ZO-1), an ~220 kDa tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.

Alternate Names

DKFZp686M05161, MGC133289, Tight junction protein 1, tight junction protein 1 (zona occludens 1), tight junction protein ZO-1, TJP1, ZO1, ZO-1, zona occludens 1, Zona occludens protein 1, zonula occludens 1 protein, Zonula occludens protein 1

Entrez Gene IDs

7082 (Human)

Gene Symbol

TJP1

UniProt

Q07157 (Human)

Additional Tight Junction Protein 1 Products

Product Documents for Tight Junction Protein 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tight Junction Protein 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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