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TIM-2 Antibody (RMT2-14) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09036

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-09036-0.025mg
NBP3-09036-0.2mg

Key Product Details

Species Reactivity

Mouse

Applications

Block/Neutralize, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG2a Lambda Clone # RMT2-14

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

This antibody was raised by immunising Sprague Dawley rats with TIM-2-Ig consisting of the extracellular domain (aa 1-230 of mouse TIM-2) and the Fc portion of mouse IgG2a, emulsified in complete Freund's adjuvant and then fusing lymph node cells from the immunized rats with P3U1 myeloma cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Lambda

Applications for TIM-2 Antibody (RMT2-14) - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional Assay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody has been used in various FACS analyses, for instance, to evaluate the expression of TIM family members on mouse mast cells (Nakae et al, 2007) and to block the H-ferritin binding to TIM-2/L5178Y cells (Kawamoto et al, 2011). This antibody has been used in FACS and immunohistochemistry analyses to show that TIM-2 is expressed on mouse fetal hepatocytes (Watanabe et al, 2007). This antibody has also been used in Western Blots and for blocking experiments in ferritin binding analysis, which suggests that Tim-2 is the H-ferritin receptor on oligodendrocytes (Todorich et al, 2008). In addition, this antibody has been used in collagen-induced arthritis (CIA) models to determine whether TIM-2 contributes to the development of T helper (Th) 1 or Th17 cells and joint inflammation (Kawamoto et al, 2011).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TIM-2

Tim-2 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-2. It is expressed on activated T cells and preferentially on differentiated Th2 cells. Sema4A is the ligand of Tim-2. A blockade of Tim-2/Tim-2 ligand interaction results in T cell hyperproliferation and Th2 cytokine production. Tim-2 plays a critical role in the development of atopic disease and other Th2-biased immune responses.

Long Name

T Cell Immunoglobulin Mucin-2

Alternate Names

TIM2

Additional TIM-2 Products

Product Documents for TIM-2 Antibody (RMT2-14) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TIM-2 Antibody (RMT2-14) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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