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TIM-4 Antibody (5G3) [Alexa Fluor® 647]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81121AF647

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-81121AF647

Key Product Details

Species Reactivity

Mouse

Applications

Block/Neutralize, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 5G3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was raised by immunising female Lewis rats (Harlan Sprague-Dawley) with Tim-4:Ig fusion protein in complete Freund's adjuvant.

Specificity

This antibody is specific for murine TIM-4, which unlike other TIM family members, is not expressed on T lymphocytes but rather found on antigen presenting cells such as lymphoid and myeloid DC's and macrophages. TIM-4 has been shown to interact with TIM-1 thereby suggesting a role of TIM-4 in the balance of Th1/Th2 regulation. Additionally TIM-4 has been shown to be a receptor for phosphatidylserine and may play a role in engulfment of apoptotic cells.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for TIM-4 Antibody (5G3) [Alexa Fluor® 647]

Product Image: TIM-4 Antibody (5G3) [Alexa Fluor® 647] [NBP2-81121AF647] - Vial of Alexa Fluor 647 conjugated antibody. Alexa Fluor 647 is optimally excited at 653 nm by the Red laser (633 or 640 nm) and has an emission maximum of 669 nm.

Applications for TIM-4 Antibody (5G3) [Alexa Fluor® 647]

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TIM-4

The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. Studies have shown that TIM 4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM 1, and that this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM 4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain (Kuchroo et al, 2003). The TIM 4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM 1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma

Long Name

T Cell Immunoglobulin Mucin-4

Alternate Names

Smuckler, TIM4, TIMD4

Gene Symbol

TIMD4

Additional TIM-4 Products

Product Documents for TIM-4 Antibody (5G3) [Alexa Fluor® 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TIM-4 Antibody (5G3) [Alexa Fluor® 647]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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