TIM-4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-80837

Novus Biologicals, part of Bio-Techne
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NB100-80837
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all TIM-4 Antibodies »

Product Specifications

Immunogen

A 16 amino acid synthetic peptide near the center of human TIM-4. The immunogen is located within amino acids 60 - 110 of TIM-4.

Epitope

Internal

Specificity

At least four isoforms of TIM-4 are known to exist; this antibody will detect all four isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TIM-4 Antibody - BSA Free

Western Blot: TIM-4 AntibodyBSA Free [NB100-80837]

Western Blot: TIM-4 AntibodyBSA Free [NB100-80837]

Western Blot: TIM-4 Antibody - BSA Free [NB100-80837] - Jurkat lysate with TIM-4 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry-Paraffin: TIM-4 Antibody - BSA Free [NB100-80837]

Immunohistochemistry-Paraffin: TIM-4 Antibody - BSA Free [NB100-80837]

Immunohistochemistry-Paraffin: TIM-4 Antibody - BSA Free [NB100-80837] - Staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NB100-80837]

Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NB100-80837]

Immunocytochemistry/Immunofluorescence: TIM-4 Antibody - BSA Free [NB100-80837] - Jurkat cells with TIM-4 antibody at 10 ug/ml.
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Applications for TIM-4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Frozen

1 - 2 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml
Application Notes
.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TIM-4

The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. Studies have shown that TIM 4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM 1, and that this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM 4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain (Kuchroo et al, 2003). The TIM 4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM 1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma

Long Name

T Cell Immunoglobulin Mucin-4

Alternate Names

Smuckler, TIM4, TIMD4

Entrez Gene IDs

91937 (Human); 276891 (Mouse)

Gene Symbol

TIMD4

UniProt

Q96H15

Additional TIM-4 Products

Product Documents for TIM-4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TIM-4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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