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TIMM23 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33019

Novus Biologicals, part of Bio-Techne
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NBP3-33019

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic peptide within human TIMM23 aa 30-80. (Uniprot: O14925)

Localization

Mitochondrion inner membrane.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TIMM23 Antibody

TIMM23 Antibody

Western Blot: TIMM23 Antibody [NBP3-33019]

Western Blot: TIMM23 Antibody [NBP3-33019] - Western blot analysis of TIMM23 on different lysates with Rabbit anti-TIMM23 antibody (NBP3-33019) at 1/1,000 dilution.

Lane 1: Hela cell lysate
Lane 2: A549 cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33019) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
TIMM23 Antibody

Immunohistochemistry: TIMM23 Antibody [NBP3-33019]

Immunohistochemistry: TIMM23 Antibody [NBP3-33019] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-TIMM23 antibody (NBP3-33019) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33019) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
TIMM23 Antibody

Immunocytochemistry/ Immunofluorescence: TIMM23 Antibody [NBP3-33019]

Immunocytochemistry/ Immunofluorescence: TIMM23 Antibody [NBP3-33019] - Immunocytochemistry analysis of A549 cells labeling TIMM23 with Rabbit anti-TIMM23 antibody (NBP3-33019) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TIMM23 antibody (NBP3-33019) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647) were used as the secondary antibody at 1/1,000 dilution.

Applications for TIMM23 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry-Paraffin

1:800

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TIMM23

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane

Alternate Names

bA592B15.7, MGC22767, PRO1197, RP11-592B15.7, TIM23

Gene Symbol

TIMM23

Additional TIMM23 Products

Product Documents for TIMM23 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TIMM23 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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