TIMM50 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1462

Novus Biologicals, part of Bio-Techne
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NB100-1462
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all TIMM50 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CDVRTVLEHYALEDD corresponding to internal region according to NP_001001563.1.

Specificity

This antibody is expected to recognise an epitope corresponding to aa 396-409 of human TIM50.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TIMM50 Antibody

Western Blot: TIMM50 Antibody [NB100-1462]

Western Blot: TIMM50 Antibody [NB100-1462]

Western Blot: TIMM50 Antibody [NB100-1462] - Staining of Human Heart lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 0.5 ug/mL. Primary incubated for 1 hour. Detected using chemiluminescence.

Applications for TIMM50 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.5 - 1.5 ug/mL
Application Notes
Western blot: Approx 39 kDa in Human Heart lysate (Calculated MW of 50.5 kDa according to NP_001001563.1) . The observed molecular weight corresponds to earlier finding in literature with different antibodies (PMID: 15044455). This protein has a calculated MW of 50.5 kDa band according to NP_001001563.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TIMM50

Tim50 is a component of the yeast Tim23 import machinery, which mediates translocation of presequence containing proteins across the mitochondrial inner membrane. By searching sequence databases, open reading frames encoding proteins with similarity to Tim50 and with a putative mitochondrial presequence were identified in the genomes of evolutionarily distant organisms, including human. Tim50 was found to interact with the N terminal intermembrane space domain of Tim23. Functional defects of Tim50 either by depletion of the protein or addition of anti Tim50 antibodies blocked the protein translocation across the inner membrane. A translocation intermediate accumulated at the translocator of the outer mitochondrial membrane (TOM) complex was cross linked to Tim50. Thus it can be concluded that Tim50, in cooperation with Tim23, facilitates transfer of the translocating protein from the TOM complex to the Tim23 complex.

Alternate Names

homolog of yeast Tim50, MGC102733, mitochondrial import inner membrane translocase subunit TIM50, TIM50, TIM50L, Tim50-like protein, translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae), translocase of inner mitochondrial membrane 50 homolog (yeast)

Entrez Gene IDs

92609 (Human)

Gene Symbol

TIMM50

UniProt

NP_001001563. (Human)

Additional TIMM50 Products

Product Documents for TIMM50 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TIMM50 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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