TIMP-3 Antibody - IHC-Prediluted

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48469

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Equine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TIMP-3 Antibodies »

Product Specifications

Immunogen

TIMP-3 (aa175-211) (Uniprot: P35625)

Reactivity Notes

The amino acid sequence used as the immunogen for the TIMP3 antibody is 100% homologous in human, cow, dog and horse, and 94% homologous in mouse and rat. Sheep data from customer review.

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for TIMP-3 Antibody - IHC-Prediluted

Immunohistochemistry-Paraffin: TIMP-3 Antibody - IHC-Prediluted [NBP2-48469]

Immunohistochemistry-Paraffin: TIMP-3 Antibody - IHC-Prediluted [NBP2-48469]

Immunohistochemistry-Paraffin: TIMP-3 Antibody - IHC-Prediluted [NBP2-48469] - TIMP-3 is expressed in first trimester human decidual cells. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: TIMP-3 Antibody - IHC-Prediluted [NBP2-48469]

Immunohistochemistry-Paraffin: TIMP-3 Antibody - IHC-Prediluted [NBP2-48469]

Immunohistochemistry-Paraffin: TIMP-3 Antibody - IHC-Prediluted [NBP2-48469] - Formalin-fixed, paraffin-embedded human placenta stained with TIMP-3 Rabbit PAb.

Applications for TIMP-3 Antibody - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TIMP-3

TIMPs form tight inhibitory complexes with MMPs, which are involved in matrix degradation and in tumor invasiveness and metastasis. TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Long Name

Tissue Inhibitors of Metalloproteinases 3

Alternate Names

TIMP3

Gene Symbol

TIMP3

Additional TIMP-3 Products

Product Documents for TIMP-3 Antibody - IHC-Prediluted

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TIMP-3 Antibody - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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