TIP60 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24613

Novus Biologicals, part of Bio-Techne
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NBP2-24613
NBP2-24613SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Canine (100%), Chicken (100%), Chimpanzee (100%), Monkey (95%), Mouse (100%), Rat (100%), Xenopus (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TIP60 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 480-530 of human TIP60 was used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen is 85% homologous in Zebrafish.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TIP60 Antibody

Western Blot: TIP60 Antibody [NBP2-24613]

Western Blot: TIP60 Antibody [NBP2-24613]

Western Blot: TIP60 Antibody [NBP2-24613] - analysis of TIP60 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain lysate using this antibody. 25 ug/ml.
Immunohistochemistry-Paraffin: TIP60 Antibody [NBP2-24613]

Immunohistochemistry-Paraffin: TIP60 Antibody [NBP2-24613]

Immunohistochemistry-Paraffin: TIP60 Antibody [NBP2-24613] - analysis of TIP60 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
Simple Western: TIP60 Antibody [NBP2-24613]

Simple Western: TIP60 Antibody [NBP2-24613]

Simple Western: TIP60 Antibody [NBP2-24613] - Simple Western lane view shows a specific band for TIP60 in 0.2 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for TIP60 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Simple Western

1:25

Western Blot

0.25-1.0 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 ml PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TIP60

HTATIP (HIV-1 Tat interacting protein TIP60, about 60kDa) belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure. There are five major variants of histone H2A: canonical H2A (most abundant), H2A.X, MacroH2A, H2ABbd and H2A.Z. Histone H2A.Z, the most conserved variant across species, functions as both a positive and negative regulator of transcription and is important for chromosome stability. Several homologous protein complexes, such as SWR-C, TIP60 and SRCAP (mammals), have been shown to catalyze the ATP-dependent exchange of H2A.Z for H2A in the nucleosome. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction.

Long Name

Histone acetyltransferase KAT5

Alternate Names

HTATIP, KAT5

Gene Symbol

KAT5

UniProt

Q92993 (Human)

Additional TIP60 Products

Product Documents for TIP60 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TIP60 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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