TIPIN Antibody (1G11)

Novus Biologicals, part of Bio-Techne | Catalog # H00054962-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TIPIN Antibodies »

Product Specifications

Immunogen

TIPIN (AAH00870, 1 a.a. ~ 301 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLEPQENGVIDLPDYEHVEDETFPPFPPPASPERQDGEGTEPDEESGNGAPVPVPPKRTVKRNIPKLDAQRLISERGLPALRHVFDKAKFKGKGHEAEDLKMLIRHMEHWAHRLFPKLQFEDFIDRVEYLGSKKEVQTCLKRIRLDLPILHEDFVSNNDEVAENNEHDVTSTELDPFLTNLSESEMFASELSRSLTEEQQQRIERNKQLALERRQAKLLSNSQTLGNDMLMNTPRAHTVEEVNTDEDQKEESNGLNEDILDNPCNDAIANTLNEEETLLDQSFKNVQQQLDATSRNITEAR

Reactivity Notes

Human. Other species not tested.

Specificity

FLJ20516 - timeless-interacting protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TIPIN Antibody (1G11)

Western Blot: TIPIN Antibody (1G11) [H00054962-M01]

Western Blot: TIPIN Antibody (1G11) [H00054962-M01]

Western Blot: TIPIN Antibody (1G11) [H00054962-M01] - Analysis of TIPIN expression in transfected 293T cell line by TIPIN monoclonal antibody (M01), clone 1G11.Lane 1: TIPIN transfected lysate(34.496 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: TIPIN Antibody (1G11) [H00054962-M01]

Immunocytochemistry/ Immunofluorescence: TIPIN Antibody (1G11) [H00054962-M01]

Immunocytochemistry/Immunofluorescence: TIPIN Antibody (1G11) [H00054962-M01] - Analysis of monoclonal antibody to TIPIN on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: TIPIN Antibody (1G11) [H00054962-M01] - Detection limit for recombinant GST tagged TIPIN is 0.03 ng/ml as a capture antibody.

Applications for TIPIN Antibody (1G11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA and immunofluorescence.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TIPIN

Timeless-interacting protein (TIPIN) was originally isolated as a protein that interacts with timeless, a factor that plays a role in circadian rhythm and cell cycle checkpoint regulation. The TIM-TIPIN complex has been shown to coordinate the intra-S-phase checkpoint in response to UV-induced DNA damage. TIPIN is required for cell cycle arrest in response to DNA damage. It also interacts with RPA (replication protein A) on DNA and is able to slow down DNA chain elongation in response to UV-damage.

Alternate Names

FLJ20516, TIMELESS interacting protein, TIMELESS-interacting protein

Entrez Gene IDs

54962 (Human)

Gene Symbol

TIPIN

UniProt

AAH00870 (Human)

Additional TIPIN Products

Product Documents for TIPIN Antibody (1G11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TIPIN Antibody (1G11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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