TIPIN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98947

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TIPIN Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human TIPIN.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for TIPIN Antibody

Western Blot: TIPIN Antibody [NBP2-98947]

Western Blot: TIPIN Antibody [NBP2-98947]

Western Blot: TIPIN Antibody [NBP2-98947] - Lane A: HCT116 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:50 kDa
Immunocytochemistry/ Immunofluorescence: TIPIN Antibody [NBP2-98947]

Immunocytochemistry/ Immunofluorescence: TIPIN Antibody [NBP2-98947]

Immunocytochemistry/Immunofluorescence: TIPIN Antibody [NBP2-98947] - Immunofluorescence staining of TIPIN in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TIPIN polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.
Immunoprecipitation: TIPIN Antibody [NBP2-98947]

Immunoprecipitation: TIPIN Antibody [NBP2-98947]

Immunoprecipitation: TIPIN Antibody [NBP2-98947] - Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-TIPIN rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TIPIN rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size :53 kDa

Applications for TIPIN Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

1-5 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TIPIN

Timeless-interacting protein (TIPIN) was originally isolated as a protein that interacts with timeless, a factor that plays a role in circadian rhythm and cell cycle checkpoint regulation. The TIM-TIPIN complex has been shown to coordinate the intra-S-phase checkpoint in response to UV-induced DNA damage. TIPIN is required for cell cycle arrest in response to DNA damage. It also interacts with RPA (replication protein A) on DNA and is able to slow down DNA chain elongation in response to UV-damage.

Alternate Names

FLJ20516, TIMELESS interacting protein, TIMELESS-interacting protein

Gene Symbol

TIPIN

Additional TIPIN Products

Product Documents for TIPIN Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TIPIN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.