TIRAP Antibody (JE46-35)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33024
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE46-35
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human TIRAP aa 1-50 / 221. (Uniprot: P58753)
Localization
Cytoplasm, Cell membrane, Membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TIRAP Antibody (JE46-35)
Western Blot: TIRAP Antibody (JE46-35) [NBP3-33024]
Western Blot: TIRAP Antibody (JE46-35) [NBP3-33024] - Western blot analysis of TIRAP on different lysates with Rabbit anti-TIRAP antibody (NBP3-33024) at 1/2,000 dilution.Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: U-2 OS cell lysate (20 ug/Lane)
Lane 3: RAW264.7 cell lysate (20 ug/Lane)
Lane 4: Mouse liver tissue lysate (40 ug/Lane)
Predicted band size: 24 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33024) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for TIRAP Antibody (JE46-35)
Application
Recommended Usage
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TIRAP
Alternate Names
adapter protein wyatt, Adaptor protein Wyatt, MAL, MyD88 adapter-like protein, MyD88-2, TIR domain-containing adapter protein, toll/interleukin-1 receptor domain-containing adapter protein, toll-interleukin 1 receptor (TIR) domain containing adaptor protein, Toll-interleukin 1 receptor (TIR) domain-containing adaptor protein, Toll-like receptor adaptor protein, wyatt
Gene Symbol
TIRAP
Additional TIRAP Products
Product Documents for TIRAP Antibody (JE46-35)
Product Specific Notices for TIRAP Antibody (JE46-35)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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