Titin Antibody (8G9)

Novus Biologicals, part of Bio-Techne | Catalog # H00007273-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 8G9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Titin Antibodies »

Product Specifications

Immunogen

TTN (AAH58824, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTTQAPTFTQPLQSVVVLEGSTATFEAHISGFPVPEVSWFRDGQVISTSTLPGVQISFSDGRAKLTIPAVTKANSGRYSLKATNGSGQATSTAELLVKAETAPPNFVQRL

Specificity

TTN - titin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Titin Antibody (8G9)

Immunocytochemistry/ Immunofluorescence: Titin Antibody (8G9) [H00007273-M01]

Immunocytochemistry/ Immunofluorescence: Titin Antibody (8G9) [H00007273-M01]

Immunocytochemistry/Immunofluorescence: Titin Antibody (8G9) [H00007273-M01] - Analysis of monoclonal antibody to TTN on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: Titin Antibody (8G9) [H00007273-M01] - Detection limit for recombinant GST tagged TTN is approximately 0.3ng/ml as a capture antibody.

Applications for Titin Antibody (8G9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Titin

This gene encodes a large abundant protein of striated muscle. The product of this gene is divided into two regions, a N-terminal I-band and a C-terminal A-band. The I-band, which is the elastic part of the molecule, contains two regions of tandem immunoglobulin domains on either side of a PEVK region that is rich in proline, glutamate, valine and lysine. The A-band, which is thought to act as a protein-ruler, contains a mixture of immunoglobulin and fibronectin repeats, and possesses kinase activity. A N-terminal Z-disc region and a C-terminal M-line region bind to the Z-line and M-line of the sarcomere respectively so that a single titin molecule spans half the length of a sarcomere. Titin also contains binding sites for muscle associated proteins so it serves as an adhesion template for the assembly of contractile machinery in muscle cells. It has also been identified as a structural protein for chromosomes. Considerable variability exists in the I-band, the M-line and the Z-disc regions of titin. Variability in the I-band region contributes to the differences in elasticity of different titin isoforms and, therefore, to the differences in elasticity of different muscle types. Of the many titin variants identified, five for which complete transcript information is available are described. Mutations in this gene are associated with familial hypertrophic cardiomyopathy 9 and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma.

Alternate Names

cardiomyopathy, dilated 1G (autosomal dominant), CMD1G, CMH9, CMPD4, Connectin, DKFZp451N061, EC 2.7.11.1, EOMFC, FLJ32040, FLJ34413, FLJ39564, FLJ43066, HMERF, LGMD2JFLJ26020, MPRM, Rhabdomyosarcoma antigen MU-RMS-40.14, titin, TMDFLJ26409

Entrez Gene IDs

7273 (Human)

Gene Symbol

TTN

OMIM

188840 (Human)

UniProt

AAH58824 (Human)

Additional Titin Products

Product Documents for Titin Antibody (8G9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Titin Antibody (8G9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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