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Titin Antibody (T11)

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1206

Novus Biologicals, part of Bio-Techne
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NB600-1206-0.025ml
NB600-1206

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Avian, Chicken, Fish, Insect, Mammal, Reptile

Cited:

Mouse, Insect

Applications

Validated:

Electron Microscopy, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # T11

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Full length native protein (purified) (Chicken breast muscle).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Insect reactivity reported in scientific literature (PMID: 29499397).

Specificity

The antibody localizes titin (connectin) in skeletal and heart muscle of a wide variety of species from cold-blooded vertebrates to human. The antibody does not cross-react with nebulin. Likewise, it does not react with smooth muscle, non-muscle tissues, or cultured cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Titin Antibody (T11)

Application
Recommended Usage

Electron Microscopy

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Titin

Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures. It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors.By indirect immunofluorescence the antibody displays a typical striated staining pattern on frozen sections of chicken skeletal and cardiac muscle tissues. Stains the region of the A-I junction by indirect immunofluorescence. It shows a decoration line 0.05 mm from the end of the A band in electron micro-graphs.In immunoblotting, using total extracts of chicken breast muscle, the antibody reacts specifically with both bands of the titin double and shows no reaction with nebulin.

Alternate Names

cardiomyopathy, dilated 1G (autosomal dominant), CMD1G, CMH9, CMPD4, Connectin, DKFZp451N061, EC 2.7.11.1, EOMFC, FLJ32040, FLJ34413, FLJ39564, FLJ43066, HMERF, LGMD2JFLJ26020, MPRM, Rhabdomyosarcoma antigen MU-RMS-40.14, titin, TMDFLJ26409

Entrez Gene IDs

7273 (Human); 22138 (Mouse); 84015 (Rat)

Gene Symbol

TTN

Additional Titin Products

Product Documents for Titin Antibody (T11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Titin Antibody (T11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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