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Titin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62600

Novus Biologicals, part of Bio-Techne
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NBP2-62600
NBP2-62600-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: HFLSILTIDTSDAEDYSCVLVEDENVKTTAKLIVEGAVVEFVKELQDIEVPESYSGELECIVSPENIEGKWYHNDVELKSNGKYTITSRRGRQNLTVKDVTKEDQGEYSFVIDGKKTTCKLKMK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Titin Antibody

Immunohistochemistry-Paraffin: Titin Antibody [NBP2-62600]

Immunohistochemistry-Paraffin: Titin Antibody [NBP2-62600]

Immunohistochemistry-Paraffin: Titin Antibody [NBP2-62600] - Staining of human skeletal muscle shows high expression.
Immunohistochemistry-Paraffin: Titin Antibody [NBP2-62600]

Immunohistochemistry-Paraffin: Titin Antibody [NBP2-62600]

Immunohistochemistry-Paraffin: Titin Antibody [NBP2-62600] - Immunohistochemistry analysis in human skeletal muscle and liver tissues using Anti-TTN antibody. Corresponding TTN RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Titin Antibody [NBP2-62600]

Immunohistochemistry-Paraffin: Titin Antibody [NBP2-62600]

Immunohistochemistry-Paraffin: Titin Antibody [NBP2-62600] - Staining of human liver shows low expression as expected.

Applications for Titin Antibody

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Titin

Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures. It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors.By indirect immunofluorescence the antibody displays a typical striated staining pattern on frozen sections of chicken skeletal and cardiac muscle tissues. Stains the region of the A-I junction by indirect immunofluorescence. It shows a decoration line 0.05 mm from the end of the A band in electron micro-graphs.In immunoblotting, using total extracts of chicken breast muscle, the antibody reacts specifically with both bands of the titin double and shows no reaction with nebulin.

Alternate Names

cardiomyopathy, dilated 1G (autosomal dominant), CMD1G, CMH9, CMPD4, Connectin, DKFZp451N061, EC 2.7.11.1, EOMFC, FLJ32040, FLJ34413, FLJ39564, FLJ43066, HMERF, LGMD2JFLJ26020, MPRM, Rhabdomyosarcoma antigen MU-RMS-40.14, titin, TMDFLJ26409

Gene Symbol

TTN

Additional Titin Products

Product Documents for Titin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Titin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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