TLE1 Antibody (TLE1/8007R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20696
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # TLE1/8007R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for TLE1 Antibody (TLE1/8007R) - Azide and BSA Free
Immunogen
Recombinant human TLE1 fragment (aa 175-338) (exact sequence is proprietary)
Localization
Nucleus.
Specificity
Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein 1 (TLE1) is a protein that is encoded by the TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: HeLa, HepG2 or Jurkat cells. Human endometrium carcinoma or sarcomas.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for TLE1 Antibody (TLE1/8007R) - Azide and BSA Free
TLE1 Antibody (TLE1/8007R)
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with TLE1 antibody (TLE1/8007R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
TLE1 Antibody (TLE1/8007R)
Formalin-fixed, paraffin-embedded human bladder stained with TLE1 antibody (TLE1/8007R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for TLE1 Antibody (TLE1/8007R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: TLE1
Long Name
Transducin-like Enhancer of Split 1
Alternate Names
GRG1
Gene Symbol
TLE1
Additional TLE1 Products
Product Documents for TLE1 Antibody (TLE1/8007R) - Azide and BSA Free
Product Specific Notices for TLE1 Antibody (TLE1/8007R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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