TLE6 Antibody (2E4)
Novus Biologicals, part of Bio-Techne | Catalog # H00079816-M01
Key Product Details
Species Reactivity
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2E4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
TLE6 (AAH20206, 1 a.a. ~ 449 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATRSSDWLRRPLGEDNQPETQLFWDKEPWFWHDTLTEQLWRIFAGVHDEKAKPRDRQQAPGLGQESKAPGSCDPGTDPCPEDASTPRPPEASSSPPEGSQDRNTSWGVVQEPPGRASRFLQSISWDPEDFEDAWKRPDALPGQSKRLAVPCKLEKMRILAHGELVLATAISSFTRHVFTCGRRGIKVWSLTGQVAEDRFPESHLPIQTPGAFLRTCLLSSNSRSLLTGGYNLASVSVWDLAAPSLHVKEQLPCAGLNCQALDANLDANLAFASFTSGVVRIWDLRDQSVVRDLKGYPDGVKSIVVKGYNIWTGGPDACLRCWDQRTIMKPLEYQFKSQIMSLSHSPQEDWVLLGMANGQQWLQSTSGSQRHMVGQKDSVILSVKFSPFGQWWASVGMDDFLGVYSMPAGTKVFEVPEMSPVTCCDVSSNNRLVVTGSGEHASVYQITY
Reactivity Notes
Human. Other species not tested.
Specificity
TLE6 - transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (2E4)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for TLE6 Antibody (2E4)
![Western Blot: TLE6 Antibody (2E4) [H00079816-M01]](https://resources.bio-techne.com/images/products/TLE6-Antibody-2E4-Western-Blot-H00079816-M01-img0001.jpg "Western Blot: TLE6 Antibody (2E4) [H00079816-M01]")
Western Blot: TLE6 Antibody (2E4) [H00079816-M01]
Western Blot: TLE6 Antibody (2E4) [H00079816-M01] - Analysis of TLE6 expression in transfected 293T cell line by TLE6 monoclonal antibody (M01), clone 2E4.Lane 1: TLE6 transfected lysate (Predicted MW: 49.8 KDa).Lane 2: Non-transfected lysate.Applications for TLE6 Antibody (2E4)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Use in Immunocytochemistry/immunofluorescence and Immunohistochemistry reported in scientific literature (PMID 24356439)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TLE6
Alternate Names
GRG6FLJ14009, MGC14966, transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila), transducin-like enhancer protein 6
Entrez Gene IDs
79816 (Human)
Gene Symbol
TLE6
UniProt
Additional TLE6 Products
Product Documents for TLE6 Antibody (2E4)
Product Specific Notices for TLE6 Antibody (2E4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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