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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-296 of human TLK2(NP_006843) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

85.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TLK2 Antibody (OTI2D10) - Azide and BSA Free

Immunohistochemistry: TLK2 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74532]

Immunohistochemistry: TLK2 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74532]

Immunohistochemistry: TLK2 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74532] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: TLK2 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74532]

Immunohistochemistry: TLK2 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74532]

Immunohistochemistry: TLK2 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74532] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TLK2 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74532]

Immunohistochemistry: TLK2 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74532]

Immunohistochemistry: TLK2 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74532] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for TLK2 Antibody (OTI2D10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TLK2

TLK2 (Tousled-like kinase 2) is a nuclear serine/threonine kinase. It is cell cycle checkpoint and ATM-pathway dependent, with maximal activity in S-phase. It is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. TLK2 is potentially involved in the regulation of chromatin assembly during DNA replication.

Alternate Names

EC 2.7.11, EC 2.7.11.1, MGC44450, PKU-ALPHA, serine/threonine-protein kinase tousled-like 2, tousled-like kinase 2serine/threonine kinase

Gene Symbol

TLK2

Additional TLK2 Products

Product Documents for TLK2 Antibody (OTI2D10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TLK2 Antibody (OTI2D10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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