TLR1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56563
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Dot Blot, Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Cytometric Bead Assay Standard, Flow Cytometry, Flow Cytometry Control, IF/IHC, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was developed against a KLH-conjugated synthetic peptide corresponding to a sequence within amino acids 400-450 of human TLR1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TLR1 Antibody
Flow Cytometry: TLR1 Antibody [NB100-56563]
Flow Cytometry: TLR1 Antibody [NB100-56563] - TLR1 expression pattern in primary BMNCs from 3 MM patients analyzed by flow cytometry. CD138-positive cells were gated from the total cell population. Staining for was compared with isotype-matched controls (NC). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.Western Blot: TLR1 Antibody [NB100-56563]
Western Blot: TLR1 Antibody [NB100-56563] - analysis of TLR1 in Ramos (A), Raw (B), and (C) TLR1 transfected cell lysate using this antibody. goat anti-rabbit HRP secondary antibody was used.Western Blot: TLR1 Antibody [NB100-56563]
Western Blot: TLR1 Antibody [NB100-56563] - All the cell lines expressed mRNA for TLR1 (Figure 1) and displayed a strong expression of TLR1 protein (Figure 2). Cell lysates were electrophoresed and blotted to PVDF membrane, which was probed with TLR1-specific antibody. To confirm the immunoreactivity of the antibody, different positive controls were included. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.Applications for TLR1 Antibody
Application
Recommended Usage
Flow (Cell Surface)
reported in scientific literature (Wong et al (2007); PMID 23593278)
Flow (Intracellular)
0.5-1 ug/1 million cells
Flow Cytometry
2-4 ug/ 1x10-6 cells
Immunohistochemistry
reported in scientific literature (PMID 27008696)
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
1-3 ug/ml
Application Notes
In Ramos, an 87 kDa band is observed. Flow (Intracellular) reported in scientific literature (PMID: 23593278)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TLR1
Long Name
Toll-like Receptor 1
Alternate Names
CD281
Gene Symbol
TLR1
UniProt
Additional TLR1 Products
Product Documents for TLR1 Antibody
Product Specific Notices for TLR1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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