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TLR1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56563

Novus Biologicals, part of Bio-Techne
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NB100-56563
NB100-56563SS

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Dot Blot, Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Cytometric Bead Assay Standard, Flow Cytometry, Flow Cytometry Control, IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

This antibody was developed against a KLH-conjugated synthetic peptide corresponding to a sequence within amino acids 400-450 of human TLR1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TLR1 Antibody

Flow Cytometry: TLR1 Antibody [NB100-56563]

Flow Cytometry: TLR1 Antibody [NB100-56563]

Flow Cytometry: TLR1 Antibody [NB100-56563] - TLR1 expression pattern in primary BMNCs from 3 MM patients analyzed by flow cytometry. CD138-positive cells were gated from the total cell population. Staining for was compared with isotype-matched controls (NC). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.
Western Blot: TLR1 Antibody [NB100-56563]

Western Blot: TLR1 Antibody [NB100-56563]

Western Blot: TLR1 Antibody [NB100-56563] - analysis of TLR1 in Ramos (A), Raw (B), and (C) TLR1 transfected cell lysate using this antibody. goat anti-rabbit HRP secondary antibody was used.
Western Blot: TLR1 Antibody [NB100-56563]

Western Blot: TLR1 Antibody [NB100-56563]

Western Blot: TLR1 Antibody [NB100-56563] - All the cell lines expressed mRNA for TLR1 (Figure 1) and displayed a strong expression of TLR1 protein (Figure 2). Cell lysates were electrophoresed and blotted to PVDF membrane, which was probed with TLR1-specific antibody. To confirm the immunoreactivity of the antibody, different positive controls were included. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.

Applications for TLR1 Antibody

Application
Recommended Usage

Flow (Cell Surface)

reported in scientific literature (Wong et al (2007); PMID 23593278)

Flow (Intracellular)

0.5-1 ug/1 million cells

Flow Cytometry

2-4 ug/ 1x10-6 cells

Immunohistochemistry

reported in scientific literature (PMID 27008696)

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1-3 ug/ml
Application Notes
In Ramos, an 87 kDa band is observed. Flow (Intracellular) reported in scientific literature (PMID: 23593278)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR1

The Toll-like receptor (TLR) family in mammals are comprised of a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. The TLRs could constitute an important and unrecognized component of innate immunity in humans. In mice, TLR1 interacts with TLR2 and coexpression of TLR1 and TLR2 enhance the NF-kappaB activation in response to a synthetic lipopeptide. They recognize the lipid configuration of the native mycobacterial lipoprotein as well as several triacylated lipopeptides.

Long Name

Toll-like Receptor 1

Alternate Names

CD281

Entrez Gene IDs

7096 (Human); 305354 (Rat)

Gene Symbol

TLR1

UniProt

Additional TLR1 Products

Product Documents for TLR1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TLR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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