Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 11G5

Format

BSA Free

Concentration

1.0 mg/ml

View all TLR2 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 400-450 of murine TLR2 was used as the immunogen for this antibody.

Reactivity Notes

The amino acid sequence used as immunogen is 80% in Rat, Chinese Hamster and Rabbit.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for TLR2 Antibody (11G5) - BSA Free

Western Blot: TLR2 Antibody (11G5)BSA Free [NBP2-27165]

Western Blot: TLR2 Antibody (11G5)BSA Free [NBP2-27165]

Western Blot: TLR2 Antibody (11G5) [NBP2-27165] - Analysis of TLR2 antibody. Mouse RAW cell lysate was probed using TLR2 antibody at 5 ug/ml. goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Flow Cytometry: TLR2 Antibody (11G5) - BSA Free [NBP2-27165]

Flow Cytometry: TLR2 Antibody (11G5) - BSA Free [NBP2-27165]

Flow Cytometry: TLR2 Antibody (11G5) [NBP2-27165] - Cell surface analysis using TLR2 antibody. Thyoglycollate-elicited peritoneal macrophages from C57BL/6 mice were tested with 2.5 ug of isotype control antibody (green) and 2.5 ug of TLR2 antibody (red).

Applications for TLR2 Antibody (11G5) - BSA Free

Application
Recommended Usage

Flow Cytometry

2.5ug/10^6cells

Western Blot

4-6ug/ml~
Application Notes
This monoclonal antibody only very weakly detects human TLR2 in Ramos cell lysate and is not recommended for human sample testing. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR2

The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ Nk cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

Long Name

Toll-like Receptor 2

Alternate Names

CD282

Gene Symbol

TLR2

UniProt

Q9QUN7

Additional TLR2 Products

Product Documents for TLR2 Antibody (11G5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TLR2 Antibody (11G5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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