TLR2 Antibody (TL2.1) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12062
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: TLR2 Antibody (TL2.1) - Chimeric - Azide and BSA Free [NBP3-12062]](https://resources.bio-techne.com/images/products/TLR2-Antibody-TL2-1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12062-img0001.jpg "Immunocytochemistry/ Immunofluorescence: TLR2 Antibody (TL2.1) - Chimeric - Azide and BSA Free [NBP3-12062]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # TL2.1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunising BALB/c mice with CHO cells transfected with human TLR2 cDNA.
Specificity
This antibody is specific to TLR2 (extracellular domain) and does not crossreact with human TLR1 or TLR4. TLR2 is involved in the immunological response against bacterial lipoproteins and other microbial cell wall components.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for TLR2 Antibody (TL2.1) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: TLR2 Antibody (TL2.1) - Chimeric - Azide and BSA Free [NBP3-12062]
Immunocytochemistry/Immunofluorescence: TLR2 Antibody (TL2.1) - Chimeric [NBP3-12062] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of TL2.1 (NBP3-12062) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12062, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications for TLR2 Antibody (TL2.1) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: TLR2
Long Name
Toll-like Receptor 2
Alternate Names
CD282
Gene Symbol
TLR2
Additional TLR2 Products
Product Documents for TLR2 Antibody (TL2.1) - Chimeric - Azide and BSA Free
Product Specific Notices for TLR2 Antibody (TL2.1) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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