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TLR2 Antibody - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24861

Novus Biologicals, part of Bio-Techne
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NBP2-24861

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

This antibody was developed against a mixture of synthetic peptides containing amino acids 180-196, 353-370, and 473-489 of human TLR2 (NP_003255).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TLR2 Antibody - Azide Free

Western Blot: TLR2 AntibodyAzide Free [NBP2-24861]

Western Blot: TLR2 AntibodyAzide Free [NBP2-24861]

Western Blot: TLR2 Antibody - Azide Free [NBP2-24861] - Analysis of TLR2 in transfected cell lysate using this antibody.
Immunohistochemistry: TLR2 Antibody - Azide Free [NBP2-24861]

Immunohistochemistry: TLR2 Antibody - Azide Free [NBP2-24861]

Immunohistochemistry: TLR2 Antibody - Azide Free [NBP2-24861] - Analysis of mouse pancreas tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml. Image using the standard format of this product.
Immunohistochemistry: TLR2 Antibody - Azide Free [NBP2-24861]

Immunohistochemistry: TLR2 Antibody - Azide Free [NBP2-24861]

Immunohistochemistry: TLR2 Antibody - Azide Free [NBP2-24861] - Representative photographs of immunohistochemistry for tubular TLR2 in G) ctrl, H) ctrl + empa, I) diabetic (dm), J) dm + empagliflozin, K) dm + telmisartan groups (Magnification = original X 200) and quantification of tubular TLR2 expression by Image J (L). (Data are expressed as mean +/- SEM with *=P<0.05 vs ctrl, **=P<0.001 vs ctrl and #=P<0.05 vs dm). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0108994) licensed under a CC-BY license. Image using the standard format of this product.

Applications for TLR2 Antibody - Azide Free

Application
Recommended Usage

Western Blot

1-3 ug/ml
Application Notes
In transfected human TLR2 cell lysate, a 90 kDa band is observed. This has also been reported to work well in bronchial epithelial cells (UBH16E) and THP-1 cells.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 ml sterile PBS

Format

Azide Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TLR2

Toll-like receptors are type I transmembrane signaling receptors which are critical for the innate host defense to pathogens. Toll-like receptor 2 (TLR2), known as CD282, has been identified as a receptor that is central to the innate immune system's response to lipoproteins of Gram-negative bacteria and Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. The TL2.1 antibody is useful for blocking studies. It has been reported to block TLR2 agonist-induced cellular activation.

Long Name

Toll-like Receptor 2

Alternate Names

CD282

Gene Symbol

TLR2

Additional TLR2 Products

Product Documents for TLR2 Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TLR2 Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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