TLR5 Antibody (19D759.2) [PE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24783

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-24783
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 19D759.2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TLR5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 between amino acids 700-800. The antibody also reacts with mouse TLR5.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for TLR5 Antibody (19D759.2) [PE]

Flow Cytometry: TLR5 Antibody (19D759.2) [PE] [NBP2-24783] - Intracellular flow analysis of TLR5 in human PBMCs using 1 ug/10^6 cells of this antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR5 antibody.
Flow Cytometry: TLR5 Antibody (19D759.2) [PE] [NBP2-24783] - Analyis of CD14-/CD83+/TLR5+ diffferentiated myeloid dendritic cells (mDCs). PBMCs were stimulated 5 days with GM-CSF+IL4 prior to the staining. The cells were stained for surface markers CD14 and CD83 FITC, and TLR5 PE. CD14- cells were gated and stained with PE-conjugated isotype control (mouse IgG2a), and FITC-conjugated CD83 (left). CD14- cells were gated and stained with 1 ug PE-conjugated TLR5 , and FITC-conjugated CD83 (right).

Applications for TLR5 Antibody (19D759.2) [PE]

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TLR5

The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

Long Name

Toll-like Receptor 5

Alternate Names

TIL-3

Gene Symbol

TLR5

Additional TLR5 Products

Product Documents for TLR5 Antibody (19D759.2) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TLR5 Antibody (19D759.2) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.