TLR7 Antibody (4G6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80982

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4G6

Format

Azide and BSA Free

Concentration

1 mg/ml

View all TLR7 Antibodies »

Product Specifications

Immunogen

A partial human TLR7 recombinant protein (amino acids 562-839) was used as the immunogen.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24126361)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TLR7 Antibody (4G6) - Azide and BSA Free

Western Blot: TLR7 Antibody (4G6)Azide and BSA Free [NBP2-80982]

Western Blot: TLR7 Antibody (4G6)Azide and BSA Free [NBP2-80982]

Western Blot: TLR7 Antibody (4G6) - Azide and BSA Free [NBP2-80982] - TLR7 antibodies tested at 2 ug/ml on recombinant partial hTLR7 protein (amino acids 562-839). NB100-56682 and NBP2-24767 immunogen sequences are not present in recombinant protein; *NB100-56588 is not recommended for use in western blot. Image using the standard format of this product.
Flow Cytometry: TLR7 Antibody (4G6) - Azide and BSA Free [NBP2-80982]

Flow Cytometry: TLR7 Antibody (4G6) - Azide and BSA Free [NBP2-80982]

Flow Cytometry: TLR7 Antibody (4G6) - Azide and BSA Free [NBP2-80982] - Analysis using the PE conjugate of NBP2-27332. Staining of TLR7 in 10^6 human BDCM cells (red) and 0.5 ug of mouse IgG1 isotype control (green). this antibody was used for this test.
Western Blot: TLR7 Antibody (4G6)Azide and BSA Free [NBP2-80982]

Western Blot: TLR7 Antibody (4G6)Azide and BSA Free [NBP2-80982]

Western Blot: TLR7 Antibody (4G6) - Azide and BSA Free [NBP2-80982] - Analysis using Azide Free version of NBP2-27332. Human TLR7 antibody in Ramos and transfected 293T lysate using TLR7 monoclonal antibody at 10 ug/ml.
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Applications for TLR7 Antibody (4G6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2ug/10^6cells

Immunocytochemistry/ Immunofluorescence

1:500-1:5000. Use reported in scientific literature (PMID 22610069)

Immunohistochemistry

reported in scientific literature (PMID 31092820)

Western Blot

10ug/ml
Application Notes
This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR7

Toll-like receptor 7 (TLR7) is a type I transmembrane protein expressed on the surface of endosomes and has a role in pathogen-associated molecular patterns (PAMPs) recognition and host defense (1-3). TLR7 is primarily expressed in the brain, placenta, spleen, stomach, and lungs (4). TLR7 recognizes microbial single stranded RNA (ssRNA), specifically guanosine and its derivatives (1-3). Human TLR7 cDNA encodes a 1049 amino acid (aa) protein with a theoretical molecular weight (MW) of 120.9 kDa (4). The TLR7 protein consists of a signal sequence, an 813 aa extracellular domain containing leucine-rich repeats (LRRs) which form a horseshoe-like shape, a 21 aa transmembrane domain, and a 189 aa cytoplasmic domain with cytosolic Toll-interleukin-1 receptor homology (TIR) domains (1,2,4). TLR7 and its fellow subfamily members, TLR8 and TLR9, possess a characteristic Z-loop between two LRRs with proteolytic Z-loop processing required for TLR activation (2). Z-loop cleavage in TLR7 allows for guanosine and uridine-rich ssRNA binding to the 1st and 2nd ligand binding site, respectively (2). The TIR domain associates with the adaptor protein myeloid differentiation primary response protein (MyD88) to initiate downstream signaling (1-3,5,6). Following activation by PAMPs, TLR7 dimerizes and bound MyD88 interacts with interleukin-1 receptor-associated kinase-4 (IRAK-4) (1,5). Together the complex recruits IRAK-1 and IRAK-2, which become phosphorylated, and interact with tumor necrosis factor receptor-associated factor 6 (TRAF6) (1,5). TRAF6 induces the activation of mitogen-activated protein kinase (MAPK), nuclear factor-kappaB (NF-kappaB), and interferon-regulatory factor 7 (IRF7), leading to interferon production and pro-inflammatory cytokine secretion associated with immune response (1,5).

While TLRs play an important role in innate immune response, dysfunction in the TLR-MyD88 signaling cascade has also been reported in various autoimmune disorders (5,6). Elevated expression of TLR7 is associated with increased risk of system lupus erythematosus (SLE), an autoimmune disease involving B cell hyperactivity (6,7). Studies involving mouse models has also found that increased TLR7 expression predisposes mice to a lupus-like disease (7). Therapeutics targeting TLR7 have been developed to either enhance or inhibit its activity depending on the circumstance. For example, TLR7 agonists such as imiquimod, resiquimod, and 852A are used to increase TLR7 activity for treatment of cancers and to fight viral infections (7,8). On the other hand, TLR7 antagonists inhibit its activation and have been developed to combat chronic immune stimulation as seen in inflammatory and autoimmune diseases (8).

References

1. Petes C, Odoardi N, Gee K. The Toll for Trafficking: Toll-Like Receptor 7 Delivery to the Endosome. Front Immunol. 2017;8:1075. https://doi.org/10.3389/fimmu.2017.01075

2. Maeda K, Akira S. TLR7 Structure: Cut in Z-Loop. Immunity. 2016;45(4):705-707. https://doi.org/10.1016/j.immuni.2016.10.003

3. Krieg AM, Vollmer J. Toll-like receptors 7, 8, and 9: linking innate immunity to autoimmunity. Immunol Rev. 2007;220:251-269. https://doi.org/10.1111/j.1600-065X.2007.00572.x

4. Uniprot (Q9NYK1)

5. Zheng C, Chen J, Chu F, Zhu J, Jin T. Inflammatory Role of TLR-MyD88 Signaling in Multiple Sclerosis. Front Mol Neurosci. 2020;12:314. https://doi.org/10.3389/fnmol.2019.00314

6. Chi H, Li C, Zhao FS, et al. Anti-tumor Activity of Toll-Like Receptor 7 Agonists. Front Pharmacol. 2017;8:304. https://doi.org/10.3389/fphar.2017.00304

7. Fillatreau S, Manfroi B, Dorner T. Toll-like receptor signalling in B cells during systemic lupus erythematosus. Nat Rev Rheumatol. 2021;17(2):98-108. https://doi.org/10.1038/s41584-020-00544-4

8. Patinote C, Karroum NB, Moarbess G, et al. Agonist and antagonist ligands of toll-like receptors 7 and 8: Ingenious tools for therapeutic purposes. Eur J Med Chem. 2020;193:112238. https://doi.org/10.1016/j.ejmech.2020.112238

Long Name

Toll-like Receptor 7

Alternate Names

toll-like receptor 7

Gene Symbol

TLR7

Additional TLR7 Products

Product Documents for TLR7 Antibody (4G6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TLR7 Antibody (4G6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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