TLR9 Antibody (26C593.2) [PE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24907

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Equine, Monkey, Primate

Cited:

Human, Mouse, Canine, Primate (Rhesus monkey)

Applications

Validated:

Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Flow (Cell Surface), Flow Cytometry, Flow Cytometry Control, Immunohistochemistry-Paraffin, Western Blot

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 26C593.2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TLR9 Antibodies »

Product Specifications

Immunogen

This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).

Reactivity Notes

Rhesus Monkey.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TLR9 Antibody (26C593.2) [PE]

Flow Cytometry: TLR9 Antibody (26C593.2) [PE] [NBP2-24907] - Expression of TLR9 protein on epithelial cells. HNEC, Detroit-562 and FaDu were stained intracellularly with PE-Ab against TLR9 (open histograms) or appropriate isotype control (shaded histograms) and analyzed by flow cytometry. Representative pictures from one out of three independent experiments are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0098239), licensed under a CC-BY license.
Flow Cytometry: TLR9 Antibody (26C593.2) [PE] [NBP2-24907] - Intracellular analysis of TLR9 in human PBMCs using 0.2 ug of this antibody. Shaded histogram represents cells without antibody; green represents a mouse IgG1-PE isotype control ; red represents anti-TLR9 antibody.
Flow Cytometry: TLR9 Antibody (26C593.2) [PE] [NBP2-24907] - Intracellular flow analysis of TLR9 in Ramos cells using 0.1 ug of antibody. Shaded histogram represents Ramos cells without antibody; green represents a mouse IgG1-PE isotype control red represents anti-TLR9 antibody.
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Applications for TLR9 Antibody (26C593.2) [PE]

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells

Immunocytochemistry/ Immunofluorescence

1:10-1:2000
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TLR9

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.

Long Name

Toll-like Receptor 9

Alternate Names

CD289

Gene Symbol

TLR9

Additional TLR9 Products

Product Documents for TLR9 Antibody (26C593.2) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TLR9 Antibody (26C593.2) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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