TLR9 Antibody (2A4C6.2E5) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31150
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ICC/IF (Negative), Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # 2A4C6.2E5
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial recombinant protein made to an internal portion of mouse TLR9 (between amino acids 500-800) [UniProt Q9EQU3]
Localization
Endoplasmic reticulum membrane, Endosome, Lysosome, Cytoplasmic vesicle/phagosome.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Scientific Data Images for TLR9 Antibody (2A4C6.2E5) - BSA Free
Western Blot: TLR9 Antibody (2A4C6.2E5)BSA Free [NBP2-31150]
Western Blot: TLR9 Antibody (2A4C6.2E5) [NBP2-31150] - Mouse Liver (lane 1), Mouse Kidney (lane 2) and Mouse Spleen (lane 3) were separated by SDS-PAGE and the protein transferred to PVDF. The membrane was then probed with anti-TLR9 at 2ug/mL and detected with an anti-rat HRP secondary antibody using chemiluminescence.Immunohistochemistry-Paraffin: TLR9 Antibody (2A4C6.2E5) - BSA Free [NBP2-31150]
Immunohistochemistry-Paraffin: TLR9 Antibody (2A4C6.2E5) [NBP2-31150] - Analysis of TLR9 protein in normal mouse epididymis section using TLR9 antibody (clone 2E5) at a concentration of 5 ug/mL. This antibody developed strong cytoplasmic staining in the pseudostratified columnar epithelial cells as well as the blood vessels of epididymis. The basal cells, the smooth muscle cells and the mature sperm cells were largely found negative for TLR9 staining.Western Blot: TLR9 Antibody (2A4C6.2E5)BSA Free [NBP2-31150]
Western Blot: TLR9 Antibody (2A4C6.2E5) [NBP2-31150] - TLR9 Antibody (2E5) [NBP2-31150] - TLR9 Antibody (2E5) [NBP2-31150] - WB analysis of partial recombinant TLR9 protein (expected molecular weight ~21kDa) with TLR9 antibody (clone 2A4C6.2E5) at a concentration of 2 ug/mL.Applications for TLR9 Antibody (2A4C6.2E5) - BSA Free
Application
Recommended Usage
Immunohistochemistry
2 ug/mL
Immunohistochemistry-Paraffin
2-5 ug/mL
Western Blot
2 ug/mL
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TLR9
Long Name
Toll-like Receptor 9
Alternate Names
CD289
Entrez Gene IDs
81897 (Mouse)
Gene Symbol
TLR9
UniProt
Additional TLR9 Products
Product Documents for TLR9 Antibody (2A4C6.2E5) - BSA Free
Product Specific Notices for TLR9 Antibody (2A4C6.2E5) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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