TLR9 Antibody (eB72-1665) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43140

Clone eB72-1665 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # eB72-1665

Format

BSA Free

Concentration

0.5 mg/ml

View all TLR9 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was Tolllike receptor 9.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for TLR9 Antibody (eB72-1665) - BSA Free

Flow Cytometry: TLR9 Antibody (eB72-1665) - BSA Free [NBP1-43140]

Flow Cytometry: TLR9 Antibody (eB72-1665) - BSA Free [NBP1-43140]

Flow Cytometry: TLR9 Antibody (eB72-1665) [NBP1-43140] - Analysis using the PE conjugate of NBP1-43140. Staining of non-transfected (left) and human CD289-transfected (right) HEK 293T cells with 1.0 ug of Rat IgG2a kappa Isotype Control PE (open histogram) or 1.0 ug of Anti-Human CD289 (TLR9) PE (filled histogram). Total viable cells were used for analysis.
Flow Cytometry: TLR9 Antibody (eB72-1665) - BSA Free [NBP1-43140]

Flow Cytometry: TLR9 Antibody (eB72-1665) - BSA Free [NBP1-43140]

Flow Cytometry: TLR9 Antibody (eB72-1665) [NBP1-43140] - HEK 293 parent cells (left panel) and 293/hTLR9 transfected cells (right panel) were fixed and permeabilized and subsequently stained with either Anti-Rat IgG FITC alone (thin line) or with 1 micrograms of Anti-Human CD289 (TLR9) Purified followed by Anti-Rat IgG FITC (thick line).
Flow Cytometry: TLR9 Antibody (eB72-1665) - BSA Free [NBP1-43140]

Flow Cytometry: TLR9 Antibody (eB72-1665) - BSA Free [NBP1-43140]

Flow Cytometry: TLR9 Antibody (eB72-1665) [NBP1-43140] - HEK 293 parent cells (left panel) and 293/hTLR9 transfected cells (right panel) were fixed and permeabilized and subsequently stained with either Anti-Rat IgG FITC alone (thin line) or with 1 micrograms of Anti-Human CD289 (TLR9) Purified followed by Anti-Rat IgG FITC (thick line).

Applications for TLR9 Antibody (eB72-1665) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug/10^6 cells in 100 ul

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Preliminary in vitro blocking assays suggested that NBP1-43140 may not be effective in blocking of CpG binding. Reported to be useful in Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections (PMID:17068481)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TLR9

eB72-1665 is generated against a portion of human toll-like receptor 9 (aa 273-288), a molecule reported to be expressed predominantly intracellularly. TLR9 is a ~115-120 kDa molecule, which mediates response to unmethylated CpG dinucleotides in bacterial DNA. CpG DNA induces a strong T-helper-1-like inflammatory response and the proliferation of TLR9-positive human B cells. When stimulated with CpG DNA, TLR9-deficient (TLR9-/-) mice lacked splenocyte proliferation, inflammatory cytokine production from macrophages, and dendritic cell maturation, as compared with normal mice. To date, at least twelve members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Members of the TLR family are involved in recognition and response to different microbial components including lipoproteins, peptidoglycans, and nucleic acids and play important roles in innate immunity and inflammation. TLR9 is not detected by flow cytometry using this antibody on lysed whole human blood and/or isolated human PBMC stained for cell surface or intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Human pDCs matured in the presence of IL-3 have been reported to stain with eB72-1665 by immunofluorescence microscopy. Human Epithelial Cell lines were also reported to stain with this mAb. Further studies are needed to determine the relationship between mRNA expression and protein detection by flow cytometry.

Long Name

Toll-like Receptor 9

Alternate Names

CD289

Gene Symbol

TLR9

UniProt

Q9NR96

Additional TLR9 Products

Product Documents for TLR9 Antibody (eB72-1665) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TLR9 Antibody (eB72-1665) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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