TLR9 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76680
Conjugate
Catalog #
Key Product Details
Validated by
Orthogonal Validation, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rat
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 15 amino acids near the center of human TLR9. The immunogen is located within amino acids 840 - 890 of TLR9. Amino Acid Squence: RGRQSGRDEDALPYD
Specificity
At least two isoforms of TLR9 are known to exist; this antibody will detect both isoforms. TLR9 antibody is predicted to not cross-react with other TLR proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TLR9 Antibody - BSA Free
Western Blot: TLR9 AntibodyBSA Free [NBP1-76680]
Western Blot: TLR9 Antibody [NBP1-76680] - (G) Levels of TLR9 (NBP1-76680), COX4 (NB110-39115), MyD88, and NF-kB protein by Western blot. (H) Relative expression of TLR9 and COX4 protein. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: TLR9 Antibody - BSA Free [NBP1-76680]
Immunocytochemistry/Immunofluorescence: TLR9 Antibody [NBP1-76680] - Jurkat cells with TLR9antibody at 2 ug/ml.Western Blot: TLR9 AntibodyBSA Free [NBP1-76680]
Western Blot: TLR9 Antibody [NBP1-76680] - Analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptideApplications for TLR9 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
2-20 ug/mL
Western Blot
1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NBP1-76680 in the following applications:
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TLR9
Additional TLR9 Products
Product Documents for TLR9 Antibody - BSA Free
Product Specific Notices for TLR9 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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