Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all TLR9 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human TLR9. The immunogen is located within amino acids 960 - 1010 of TLR9. Amino Acid Squence: LSPDGRRSRYVRLRQR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TLR9 Antibody - BSA Free

Western Blot: TLR9 AntibodyBSA Free [NBP1-77254]

Western Blot: TLR9 AntibodyBSA Free [NBP1-77254]

Western Blot: TLR9 Antibody [NBP1-77254] - Mouse spleen cell lysate with TLR9 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
TLR9 Antibody - BSA Free

Immunohistochemistry: TLR9 Antibody - BSA Free [NBP1-77254] -

Immunohistochemistry: TLR9 Antibody - BSA Free [NBP1-77254] - Immunohistochemistry of TLR9 in mouse spleen cells with TLR9 antibody at 2 u/mL.
TLR9 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TLR9 Antibody - BSA Free [NBP1-77254] -

Immunocytochemistry/ Immunofluorescence: TLR9 Antibody - BSA Free [NBP1-77254] - Immunofluorescence of TLR9 in mouse spleen cells with TLR9 antibody at 10 ug/mL.

Applications for TLR9 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

0.5-1 ug/ml

Reviewed Applications

Read 1 review rated 3 using NBP1-77254 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR9

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.

Long Name

Toll-like Receptor 9

Alternate Names

CD289

Gene Symbol

TLR9

UniProt

AAH32713 (Human)

Additional TLR9 Products

Product Documents for TLR9 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TLR9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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