TMED9 Antibody (A9A8-R)

Western Blot: TMED9 Antibody (A9A8-R) [NBP3-33028]

Western Blot: TMED9 Antibody (A9A8-R) [NBP3-33028] - Western blot analysis of TMED9 on different lysates with Mouse anti-TMED9 antibody (NBP3-33028) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: THP-1 cell lysate (20 ug/Lane)
Lane 3: A549 cell lysate (20 ug/Lane)
Lane 4: MCF7 cell lysate (20 ug/Lane)
Lane 5: HepG2 cell lysate (20 ug/Lane)
Lane 6: PC-12 cell lysate (20 ug/Lane)
Lane 7: NIH/3T3 cell lysate (20 ug/Lane)
Lane 8: Mouse liver tissue lysate (40 ug/Lane)
Lane 9: Rat liver tissue lysate (40 ug/Lane)

Predicted band size: 27 kDa
Observed band size: 24 kDa

Exposure time: 43 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33028) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: TMED9 Antibody (A9A8-R) [NBP3-33028]

Immunohistochemistry: TMED9 Antibody (A9A8-R) [NBP3-33028] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-TMED9 antibody (NBP3-33028) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33028) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: TMED9 Antibody (A9A8-R) [NBP3-33028]

Immunocytochemistry/ Immunofluorescence: TMED9 Antibody (A9A8-R) [NBP3-33028] - Immunocytochemistry analysis of HepG2 cells labeling TMED9 with Mouse anti-TMED9 antibody (NBP3-33028) at 1/100 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-TMED9 antibody (NBP3-33028) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.