TMEM1 Antibody (5B4)

Novus Biologicals, part of Bio-Techne | Catalog # H00007109-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

ELISA, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5B4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TMEM1 Antibodies »

Product Specifications

Immunogen

TMEM1 (NP_003265, 1162 a.a. ~ 1257 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VRLFKYLPHHSAHSSQLDADSWIENDSLSVDKHGDDQPDSSSLKSRGSVHSACSSEHKGLPMPRLQALPAGQVFNSSSGTQVLVIPSQDDHVLEVS

Specificity

TMEM1 (5B4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TMEM1 Antibody (5B4)

Western Blot: TMEM1 Antibody (5B4) [H00007109-M01]

Western Blot: TMEM1 Antibody (5B4) [H00007109-M01]

Western Blot: TMEM1 Antibody (5B4) [H00007109-M01] - TMEM1 monoclonal antibody (M01), clone 5B4 Analysis of TMEM1 expression in Hela S3 NE.
Western Blot: TMEM1 Antibody (5B4) [H00007109-M01]

Western Blot: TMEM1 Antibody (5B4) [H00007109-M01]

Western Blot: TMEM1 Antibody (5B4) [H00007109-M01] - Analysis of TMEM1 over-expressed 293 cell line, cotransfected with TMEM1 Validated Chimera RNAi ( Cat # H00007109-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TMEM1 monoclonal antibody (M01), clone 5B4 (Cat # H00007109-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: TMEM1 Antibody (5B4) [H00007109-M01]

Western Blot: TMEM1 Antibody (5B4) [H00007109-M01]

Western Blot: TMEM1 Antibody (5B4) [H00007109-M01] - Analysis of TMEM1 expression in transfected 293T cell line by TMEM1 monoclonal antibody (M01), clone 5B4.Lane 1: TMEM1 transfected lysate(142.2 KDa).Lane 2: Non-transfected lysate.

Applications for TMEM1 Antibody (5B4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TMEM1

The protein encoded by this gene is a transmembrane protein found in the cis-Golgi complex. The encoded protein is part of the multisubunit transport protein particle (TRAPP) complex and may be involved in vesicular transport from the endoplasmic reticulum to the Golgi. Mutations in this gene could be responsible for the Unverricht-Lundborg type of progressive myoclonus epilepsy, or for autoimmune polyglandular disease type 1.

Alternate Names

EHOC-1FLJ55683, EHOC1GT334, Epilepsy holoprosencephaly candidate 1 protein, epilepsy holoprosencephaly candidate-1 protein, FLJ54223, FLJ54817, Protein GT334, TMEM1MGC126777, trafficking protein particle complex 10, trafficking protein particle complex subunit 10, trafficking protein particle complex subunit 130, Trafficking protein particle complex subunit TMEM1, transmembrane protein 1, Transport protein particle subunit TMEM1, TRAPP 130 kDa subunit, TRAPP subunit TMEM1, TRS130, TRS30

Entrez Gene IDs

7109 (Human)

Gene Symbol

TRAPPC10

OMIM

602103 (Human)

UniProt

NP_003265 (Human)

Additional TMEM1 Products

Product Documents for TMEM1 Antibody (5B4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TMEM1 Antibody (5B4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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