TMEM53 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82124

Novus Biologicals, part of Bio-Techne
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NBP1-82124
NBP1-82124-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TMEM53 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FHTHFYDRLQDAGSRWPELYLYSRADEVVLARDIERMVEARLARRVLARSVDFVSSAHVSHLRDYPTYYTSLCVDFMRNCVRC

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TMEM53 Antibody

Western Blot: TMEM53 Antibody [NBP1-82124]

Western Blot: TMEM53 Antibody [NBP1-82124]

Western Blot: TMEM53 Antibody [NBP1-82124] - Analysis in control (vector only transfected HEK293T lysate) and TMEM53 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: TMEM53 Antibody [NBP1-82124]

Immunocytochemistry/ Immunofluorescence: TMEM53 Antibody [NBP1-82124]

Immunocytochemistry/Immunofluorescence: TMEM53 Antibody [NBP1-82124] - Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & focal adhesion sites.
Immunohistochemistry: TMEM53 Antibody [NBP1-82124]

Immunohistochemistry: TMEM53 Antibody [NBP1-82124]

Immunohistochemistry: TMEM53 Antibody [NBP1-82124] - Staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.

Applications for TMEM53 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TMEM53

Alternate Names

FLJ22353, NET4, novel DUF829 domain-containing protein, transmembrane protein 53

Gene Symbol

TMEM53

Additional TMEM53 Products

Product Documents for TMEM53 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TMEM53 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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