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TMEM74 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83546

Novus Biologicals, part of Bio-Techne
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NBP1-83546
NBP1-83546-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HYLAKKSNQADLCDARDWSSRGLPGDQADTAATRAALCCQKQCASTPRATEMEGSKLSSSPASPSSSLQNSTLQPDAFPPGLLHSGNNQITAERKVCNCCSQELETSFTYVDKNINLEQRNRSSPSAKGHNHPGELGWENPNEW

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TMEM74 Antibody

Western Blot: TMEM74 Antibody [NBP1-83546]

Western Blot: TMEM74 Antibody [NBP1-83546]

Western Blot: TMEM74 Antibody [NBP1-83546] - Analysis in control (vector only transfected HEK293T lysate) and TMEM74 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: TMEM74 Antibody [NBP1-83546]

Immunohistochemistry-Paraffin: TMEM74 Antibody [NBP1-83546]

Immunohistochemistry-Paraffin: TMEM74 Antibody [NBP1-83546] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Applications for TMEM74 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TMEM74

TMEM74 plays an essential role in autophagy. TMEM74-induced autophagy may involve PI3K signal transduction

Alternate Names

FLJ30668, NET36, transmembrane protein 74

Gene Symbol

TMEM74

Additional TMEM74 Products

Product Documents for TMEM74 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TMEM74 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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